Lab 10: Biology PCR Lab Worksheet

Instructions: Answer the questions below, based on the three experiments for this lab.

Experiment 1: PCR

Which method is used to remove DNA from a cell?

How many cycles of PCR did you run your DNA through?

Experiment 2: Electrophoresis

Fill in the table below with the distance that each of the fragments in the DNA ladder traveled.
Fragment size (kbp) Distance traveled (mm)

What was the size of your amplified DNA sample fragment in kbp?

 

How far did your sample DNA PCR product travel on the gel?

What temperatures are involved in the PCR cycle? What processes take place at each temperature?

What is DNA sequencing? What does DNA sequencing tell us about an organism?

How does gel electrophoresis sort DNA fragments?

Which DNA technique would you use to insert your PCR product into another organism’s DNA?