Clinical scenario
A patient is being prepped for immediate surgery. Blood will be needed for a transfusion. The patient’s blood type is B+. However, the blood bank is out of compatible donor blood. Four nurses volunteer to donate blood so the blood bank has to determine whose blood can be used.

Introduction:
General background about blood types (ABO and Rh) noting antigens and antibodies for each
Brief explanation of how the testing works

Materials used:
4 3-well blood typing trays
4 volunteer “blood” samples (Brown, Smith, Jones, Green)
Anti-A antibody
Anti-B antibody
Anti-Rh antibody
12 stirring sticks

Procedure:
For each volunteer “blood” sample, put 2 drops of blood into three wells of one blood typing tray
Put two drops of Anti-A, Anti-B, and Anti-Rh antibodies in to the corresponding wells
Gently stir each well with a separate stick to prevent cross contamination
Agglutination (clumping) signifies a positive result, no agglutination is negative

Data:
Anti-A Anti-B Anti-Rh
Jones Neg Pos Neg
Smith Pos Neg Pos
Green Pos Pos Pos
Brown Neg Neg Neg

Conclusion:
Determine the blood types of the four volunteers and
Determine whose blood can be donated to the patient and explain why the others can not be used.