Biology Report
The data you will need to answer the following questions are listed in Table 1.
Table 1: Color, estimated pH and absorption every 10 min from the bicarbonate solution.
| Time (min) | W Syringe | G Syringe | ||||
| Color | Estimated pH | Absorption Å | Color | Estimated pH | Absorption Å | |
| 0
|
Red/Purple | 8.8 | 0.779 | Red/Purple | 8.8 | 0.761 |
| 10
|
Red/Purple | 8.8 | 0.734 | Red | 8.6 | 0.624 |
| 20
|
Red/Purple | 8.8 | 0.751 | Red | 8.6 | 0.603 |
| 30
|
Red/Purple | 8.8 | 0.753 | Dark 0range | 8.4 | 0.583 |
| 40
|
Red/Purple | 8.8 | 0.753 | Dark orange | 8.4 | 0.504 |
| 50
|
Red/Purple | 8.8 | 0.751 | Orange | 8.2 | 0.433 |
| Time (min) | Mg Syringe | ||
| Color | Estimated pH | Absorption Å
|
|
| 0
|
Red/Purple | 8.8 | 0.729 |
| 10
|
Red | 8.6 | 0.607 |
| 20
|
Dark 0range | 8.4 | 0.529 |
| 30
|
Orange | 8.2 | 0.407 |
| 40
|
Orange/Yellow | 8.2 | 0.334 |
| 50
|
Orange/Yellow | 7.8 | 0.308 |
2) (2 pnts) Calculate the change in absorption using the formula ABS0 – ABST (Absorption at time 0 min – the Absorption at time T min) and place the values in Table 2.
Table 2: Change in absorption over time for the bicarbonate solutions.
| Time (Min) | W Syringe | G Syringe | Mg Syringe |
| 0
|
0 | 0 | 0 |
| 10
|
|||
| 20
|
|||
| 30
|
|||
| 40
|
|||
| 50
|
3) (4pnts) Graph (use scatter plot) the change in absorption over time for all 3 treatments (W, G, Mg) and paste the graph below. Be sure to label the axes.
4) (3pnts) Which reaction produced CO2 the fastest? Use the rate of color change and the change in absorption to answer the question.
Data collected from conducting the Benedicts test are listed in Table 3.
Table 3: Results of Benedicts test
| Treatment | Time 0 Absorption Å | Time 0 Color | Time 50 Absorption Å | Time 50 Color |
| W
|
0.021 | Blue | 0.022 | Blue |
| G
|
0.593 | Orange/Red | 0.379 | Yellow/Orange |
| Mg
|
0.589 | Orange/Red | 0.182 | Green/yellow |
6) (1pnts) Calculate the change in absorbance between Time 0 and Time 50 from the Benedicts test and place the results in Table 4.
Table 4: Change in absorbance in the Benedicts test
| Treatment | Time 0 – Time 50 Å
|
| W
|
|
| G
|
|
| Mg
|
7) (4pnts) Which reaction showed the loss of most of the glucose? Justify your answer with the data you collected.
8) (4pnts) Based on the both data sets (bicarbonate and Benedicts) what affect did Mg have on the rate of the reaction? Justify your answer with both data sets. (Note: if you know there was an issue with some of your data you can explain what you expected to happen and why your results do not match the expectation).
